Epidemiology, burden and seasonal variation of fasciolosis determined through faecal examination and excretory/secretory antigens based ELISA

The study was conducted to evaluate predominance and the related risk factors of sheep fasciolosis in Faisalabad district, Punjab, Pakistan. In addition, comparison coprological examinationand excretory/secretory antigens (ES Ag)-based ELISA also performed determine variation prevalence fasciolosis. Ofthe1200 faecal blood samples,128 (10.67%) 241 (20.08%) samples were found positive for Fasciola infection anti-Fasciola antibodies respectively.The significantly (P < 0.05) higher female adultanimals. Most animals showed moderate (55.47%) which than mild (28.91%) severe (15.63%) infections. highest determined December (26.00% through examination; 42.00% ELISA) with a mean EPG 842.3 while lowest May (02.00% 07.00% 650. frequency distribution winter (faecal examination 20.67%; 34.67%) followed order by monsoon 09.00%; 18.67%), spring 08.33%; 17.33%) summer 04.67%; 09.67%). (894) (851.67), (654.67) (616.33). Although is gold standard “best method” diagnosis fasciolosis; however, serological tests should not be excluded especially home-made ES Ag-based might preferred more affordable assessment tool field epidemiology.